Query (optional)   in Class  All Allele Author Colleague Collection Assembly Gene Gene Class Gene Product Germplasm Image Journal Keyword Library Locus Map Map Data Marker Pathology Polymorphism Probe Protein QTL Rearrangement Reference Sequence Species Trait Trait Study Two Point Data  

---

GrainGenes Probe Report: WMC354

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC354
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 19d3 Donor: Pioneer Trimed to insert at both ends. Bases 53 to 80 show homology to 3b11 bases 95 to 122 (28 in longest run, maxScore = 41), zone is 3 - 93. Forward primer has 4 mismatches to 3b11, reverse primer is outside of the zone of homology. (GT)12 93 to 116. Bases 128 to 155 show homology to 24g3 bases 34 to 61 (complement) (28 in longest run, maxScore = 46).
Type	PCR SSR
PCR primers	Forward cAATggAgTccTTgTggTgg at 26 length 20 bases, Tm 63 Reverse AgcATTTcAcTcccgcgTAg at 166 length 20 bases, Tm 64
SSR size	160 bases expected, Chinese Spring
Amplification Conditions	61C anneal, product; 61C anneal, polymorphic on acrylamide.
Sequence	WMC354
Source Species	Triticum aestivum