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GrainGenes Probe Report: WMC358

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC358
Locus	Xwmc358-2A
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 19g4 Donor: Pioneer Trimmed to insert at both ends. Bases 61 to 113 (complement) show homology to 11d10 bases 103 to 155 (53 in longest run, maxScore = 79), zone is 44-214. Primers are outside of this region. (GT)9 175 to 192, plus (GA)3G(GA)7. Bases 56 to 72 show homology to 14a9 bases 99 to 115 (complement) (17 in longest run, maxScore = 47).
Type	PCR SSR
PCR primers	Forward TgcTAgTAgggAgAAggAgTTT at 7 length 22 bases, Tm 58 Reverse ccTTTTgTTTTgAgATTTcgcT at 214 length 22 bases, Tm 61
SSR size	229 bases expected, Chinese Spring
Amplification Conditions	51C anneal, product.
Sequence	WMC358
Source Species	Triticum aestivum