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GrainGenes Probe Report: WMC359

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC359
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 19h4 Donor: Pioneer Trimmed to insert at both ends (NB 3' linker corrupted). Bases 78 to 100 (complement) show homology to 1C2 bases 783 to 805 (23 in longest run, maxScore = 20), zone is 77-134. Reverse primer has 2 mismatches to 1c2, reverse primer is outside of this homology. (CA)25 38 to 87.
Type	PCR SSR
PCR primers	Forward TTgcAcATTcAcAccTcTccAT at 10 length 22 bases, Tm Reverse TgTTgTggcAgAAgggcA at 100 length 18 bases, Tm 65
SSR size	108 bases expected, Chinese Spring
Amplification Conditions	61C anneal, product.
Sequence	WMC359
Source Species	Triticum aestivum