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GrainGenes Probe Report: WMC361

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC361  [ Marker Report ]
Locus	Xwmc361-2B Xwmc361 WMC361 wmc361 wmc361-2B
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 19e6 Donor: Pioneer Location from nullisomic/tetrasomic (Daryl Somers, AgCanada, Feb 2001) : 221 bases located on 2B Trimmed to insert at both ends. (CA)10 327 to 346.
Type	PCR SSR
PCR primers	Forward AATgAAgATgcAAATcgAcggc at 214 length 22 bases, Tm 67 Reverse ATTcTcgcAcTgAAAAcAgggg at 408 length 22 bases, Tm 66
SSR size	216 bases expected, Chinese Spring
Amplification Conditions	61C anneal, product; 61C anneal, polymorphic on acrylamide.
Sequence	WMC361
Source Species	Triticum aestivum