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GrainGenes Probe Report: WMC362

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC362
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 19f6 Donor: Pioneer Trimmed to insert at both ends. (GT)44 90 to 177. Software struggled to get these primers.
Type	PCR SSR
PCR primers	Forward TcTATAATAATATgTATggTgTTcA at 21 length 25 bases, Tm 52 Reverse TAgcTTcATgTTAcAAAcTAATcTc at 179 length 25 bases, Tm 56
SSR size	183 bases expected, Chinese Spring
Amplification Conditions	51C anneal, product; 51C anneal, polymorphic on acrylamide.
Sequence	WMC362
Source Species	Triticum aestivum