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GrainGenes Probe Report: WMC371

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC371  [ Marker Report ]
Locus	Xwmc371-5A
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 12b10 Donor: Mohler, Munich Location from nullisomic/tetrasomic (Daryl Somers, AgCanada, Feb 2001) : 155 bases located on 5B, 5D Trimmed to insert at 5' end and to Rsa1 at 3' end. Bases 79 to 96 show homology to 11b5 bases 170 to 187 (18 in longest run, maxScore = 27). Small microsatellite (GGT)6 131 to 148, several other small GGT and GGC repeats. Bases 141 to 149 show homology to 14f8 bases 269 to 277 (9 in longest run, maxScore = 20).
Type	PCR SSR
PCR primers	Forward ggAAAccAAggcAgcAgTcA at 51 length 20 bases, Tm 65 Reverse ccAgggcTAcTTcAgccAgg at 219 length 20 bases, Tm 66
SSR size	188 bases expected, Chinese Spring
Amplification Conditions	61C anneal, product.
Sequence	WMC371
Source Species	Triticum aestivum