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GrainGenes Probe Report: WMC372

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC372
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 12c10.1 Donor: Mohler, Munich Trimmed to insert at both ends. Multiple inserts in this clone, this is the first insert. Bases 43 to 62 show homology to 1f7 bases 49 to 68 (20 in longest run, maxScore = 32). (CA)8 109 to 124, (CT)10 89 to 108.
Type	PCR SSR
PCR primers	Forward gATcAgATAAccccgAcTAccg at 63 length 22 bases, Tm 63 Reverse gTAgTcAggAAggggAgTggAA at 149 length 22 bases, Tm 63
SSR size	108 bases expected, Chinese Spring
Amplification Conditions	61C anneal, product;
Sequence	WMC372
Source Species	Triticum aestivum