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GrainGenes Probe Report: WMC374

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC374
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 12d11 Donor: Mohler, Munich Trimmed to insert at both ends. Bases 1 to 59 show homology to 14c4 bases 356 to 414 (59 in longest run, maxScore = 95). (TC)4 162 to 169, (TC)6 172 to 183, (TC)6 186 to 197, (CA)4 215 to 222, (CA)5 225 to 234.
Type	PCR SSR
PCR primers	Forward AcgAATTgAgAgAgATggTT at 123 length 20 bases, Tm 56 Reverse cAATAcgTTgTgTATAcATgAgA at 255 length 23 bases, Tm 55
SSR size	155 bases expected, Chinese Spring
Amplification Conditions	51C anneal, smear.
Sequence	WMC374
Source Species	Triticum aestivum