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GrainGenes Probe Report: WMC379

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC379  [ Marker Report ]
Locus	Xwmc379-1
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 12h12 Donor: Mohler, Munich Trimmed to Rsa1 at both ends. Bases 80 to 179 (complement) show homology to 9f3.1 bases 222 to 321 (100 in longest run, maxScore = 104). (CT)15 283 to 312, (GT)5 315 to 324.
Type	PCR SSR
PCR primers	Forward AcAAAccTTgTTggTcccTc at 249 length 20 bases, Tm 61 Reverse gAgAAccATAcATAgTgTgcgA at 375 length 22 bases, Tm 60
SSR size	148 bases expected, Chinese Spring
Amplification Conditions	61C anneal, smear.
Sequence	WMC379
Source Species	Triticum aestivum