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GrainGenes Probe Report: WMC383

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Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.


Probe
WMC383
Reference
ReferenceSomers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks
Wheat Microsatellite Consortium Project 1, Plate reference 10h8
Donor: Jacquemin, Gembloux
Trimmed to Rsa1 at 5' end and to insert at 3' end. (GT)15 315 to 344.
Type
PCR
SSR
PCR primers
Forward AgccAAAcAcTcgcATcAcg at 169 length 20 bases, Tm 66
Reverse gAAccccTTgAcAgTTcgcc at 380 length 20 bases, Tm 66
SSR size
231 bases expected, Chinese Spring
Amplification Conditions
61C anneal, product.
Sequence
WMC383
Source Species
Triticum aestivum