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GrainGenes Probe Report: WMC387

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC387  [ Marker Report ]
Locus	Xwmc387
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 10g9 Donor: Jacquemin, Gembloux Trimmed to insert at 5' end and to Rsa1 at 3' end. Bases 70 to 102 (complement) show homology to 14c4 bases 346 to 378 (33 in longest run, maxScore = 68). (CA)12 116 to 139.
Type	PCR SSR
PCR primers	Forward cATTTTgAcAcccAcAcTcg at 14 length 20 bases, Tm 62 Reverse cTggATccccTcTTcgcTAT at 189 length 20 bases, Tm 62
SSR size	195 bases expected, Chinese Spring
Amplification Conditions	51C anneal, very complex product.
Sequence	WMC387
Source Species	Triticum aestivum