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GrainGenes Probe Report: WMC389

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC389
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 10a10 Donor: Jacquemin, Gembloux Trimmed to insert at 5' end and to Rsa1 at 3' end. Bases 177 to 205 show homology to 2h10 bases 449 to 477 (29 in longest run, maxScore = 36). (GT)27 28 to 81, (GT)9 136 to 153. Bases 127 to 142 show homology to 22d1 bases 108 to 123 (complement) (16 in longest run, maxScore = 25).
Type	PCR SSR
PCR primers	Forward gTgcggggTTAgAcTcAcAAA at 3 length 21 bases, Tm 64 Reverse cAAcTgggggTTcTcAAcAAA at 155 length 21 bases, Tm 64
SSR size	173 bases expected, Chinese Spring
Amplification Conditions	61C anneal, smear.
Sequence	WMC389
Source Species	Triticum aestivum