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GrainGenes Probe Report: WMC393

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC393
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 10h11 Donor: Jacquemin, Gembloux Trimmed to insert at 5' end, 3' end not located. Bases 78 to 113 (complement) show homology to 7h5 bases 213 to 248 (36 in longest run, maxScore = 94). (CA)23 31 to 76, (CT)8 139 to 154. Too difficult to make primer.
Type	PCR SSR
PCR primers	Forward cccccTAATcTAggAccccc at 10 length 20 bases, Tm 64 Reverse gcTccgccTAgAgcATgTgT at 186 length 20 bases, Tm 65
SSR size	196 bases expected, Chinese Spring
Amplification Conditions	61C anneal, complex product.
Sequence	WMC393
Source Species	Triticum aestivum