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GrainGenes Probe Report: WMC394

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC394
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 10d12 Donor: Jacquemin, Gembloux 5' end not located, trimmed to insert at 3' end. Bases 359 to 372 (complement) show homology to 14e5 bases 80 to 93 (14 in longest run, maxScore = 28). (CA)8 314 to 329, (CA)6 331 to 342, (TA)4 343 to 350.
Type	PCR SSR
PCR primers	Forward TTTcAgTTcAATTAcTAggcAA at 239 length 22 bases, Tm 56 Reverse AAgAATAATTATTccTcAcccA at 372 length 22 bases, Tm 56
SSR size	155 bases expected, Chinese Spring
Amplification Conditions	51C anneal, product.
Sequence	WMC394
Source Species	Triticum aestivum