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GrainGenes Probe Report: WMC396

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC396  [ Marker Report ]
Locus	Xwmc396-7B Xwmc396 Stb13 wmc396-7B wmc396
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 10g12 Donor: Jacquemin, Gembloux Location from nullisomic/tetrasomic (Daryl Somers, AgCanada, Feb 2001) : 149 bases located on 7B Trimmed to Rsa1 at 5' end, 3' end not located. (GT)14 122 to 149. Bases 73 to 250 show homology to 14h11 bases 1 to 178 (complement) (178 in longest run, maxScore = 119). Duplicate of 14h11, 10g12 has priority.
Type	PCR SSR
PCR primers	Forward TgcAcTgTTTTAccTTcAcggA at 27 length 22 bases, Tm 65 Reverse cAAAgcAAgAAccAgAgccAcT at 151 length 22 bases, Tm 65
SSR size	146 bases expected, Chinese Spring
Amplification Conditions	61C anneal, polymorphic on acrylamide.
Sequence	WMC396
Source Species	Triticum aestivum