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GrainGenes Probe Report: WMC398

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC398  [ Marker Report ]
Locus	Xwmc398b-6A [ Show all 8 ]
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 22c7 Donor: Romestant, RAGT Location from nullisomic/tetrasomic (Daryl Somers, AgCanada, Feb 2001) : 156 bases located on 6B Trimmed to insert at 5' end, corrupted 3' end removed as cleanly as possible. Bases 149 to 198 show homology to 11a8 bases 348 to 397 (50 in longest run, maxScore = 42). (GT)14 32 to 59. Note that the 3' primer is located close to corrupted sequence of vector.
Type	PCR SSR
PCR primers	Forward ggAgATTgAccgAgTggAT at 1 length 19 bases, Tm 60 Reverse cgTgAgAgcggTTcTTTg at 136 length 18 bases, Tm 60
SSR size	152 bases expected, Chinese Spring
Amplification Conditions	61C anneal, polymorphic on acrylamide.
Sequence	WMC398
Source Species	Triticum aestivum