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GrainGenes Probe Report: WMC400

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC400
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 22a9 Donor: Romestant, RAGT Reverse sequence only. Trimmed to insert at 3' end, 5' end not located. Bases 121 to 146 (complement) show homology to 5e3 bases 168 to 193 (26 in longest run, maxScore = 25). (CA)21 56 to 97, (CT)9 26 to 43,
Type	PCR SSR
PCR primers	Forward TgTTcgATgTTgTgcTATgTcTgT at 2 length 24 bases, Tm 64 Reverse cTTggATggggATgAATTgTg at 124 length 21 bases, Tm 64
SSR size	143 bases expected, Chinese Spring
Amplification Conditions	51C anneal, complex product.
Sequence	WMC400
Source Species	Triticum aestivum