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GrainGenes Probe Report: WMC405

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC405  [ Marker Report ]
Locus	Xwmc405 [ Show all 20 ]
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 22d11 Donor: Romestant, RAGT Location from nullisomic/tetrasomic (Daryl Somers, AgCanada, Feb 2001) : 234 bases located on 7A Trimmed to insert at 5' end and to Ssp1 at 3' end. Bases 118 to 133 show homology to 2a9 bases 852 to 867 (16 in longest run, maxScore = 20). (CA)21 133 to 174, (CT)9 115 to 132.
Type	PCR SSR
PCR primers	Forward gTgcggAAAgAgAcgAggTT at 41 length 20 bases, Tm 63 Reverse TATgTccAcgTTggcAgAgg at 239 length 20 bases, Tm 64
SSR size	218 bases expected, Chinese Spring
Amplification Conditions	61C anneal, polymorphic product on agarose; 61C anneal, polymorphic on acrylamide.
Sequence	WMC405
Source Species	Triticum aestivum