Query (optional)   in Class  All Allele Author Colleague Collection Assembly Gene Gene Class Gene Product Germplasm Image Journal Keyword Library Locus Map Map Data Marker Pathology Polymorphism Probe Protein QTL Rearrangement Reference Sequence Species Trait Trait Study Two Point Data  

---

GrainGenes Probe Report: WMC406

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC406  [ Marker Report ]
Locus	Xwmc406-1B Xwmc406 wmc406 wmc406-1B
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 22b12 Donor: Romestant, RAGT Location from nullisomic/tetrasomic (Daryl Somers, AgCanada, Feb 2001) : 227 bases located on 1B Trimmed to Ssp1 at both ends of insert. (CA)16 188 to 219.
Type	PCR SSR
PCR primers	Forward TATgAgggTcggATcAATAcAA at 116 length 22 bases, Tm 61 Reverse cgAgTTTAcTgcAAAcAAATgg at 311 length 22 bases, Tm 61
SSR size	217 bases expected, Chinese Spring
Amplification Conditions	61C anneal, polymorphic on acrylamide (though weak).
Sequence	WMC406
Source Species	Triticum aestivum