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GrainGenes Probe Report: WMC408

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC408
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 10h2 Donor: Gale, John Innes Trimmed to Rsa1 at 5' end and to insert at 3' end. Bases 68 to 101 show homology to 9h4 bases 37 to 70 (34 in longest run, maxScore = 26). (CA)29 44 to 101. Bases 43 to 232 show homology to 25c5.2 bases 87 to 276 (190 in longest run, maxScore = 102). Duplicate with 25c5.2, 10h2 has priority.
Type	PCR SSR
PCR primers	Forward TAcgTcgATgTgTcgcTcTc at 2 length 20 bases, Tm 62 Reverse ATcTcAcTTccTggcTTccc at 173 length 20 bases, Tm 62
SSR size	191 bases expected, Chinese Spring
Amplification Conditions	61C anneal, smear (100-140 bases).
Sequence	WMC408
Source Species	Triticum aestivum