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GrainGenes Probe Report: WMC411

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC411
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 10g5 Donor: Gale, John Innes Trimmed to insert at 5' end and to Rsa1 at 3' end. Bases 10 to 36 show homology to 10g6 bases 13 to 39 (27 in longest run, maxScore = 48). (GT)10 69 to 88, (GT)15 91 to 120.
Type	PCR SSR
PCR primers	Forward gAAcccgTcAcTggAAAcATT at 41 length 21 bases, Tm 63 Reverse ccTcgTATTAccATccAgccT at 264 length 21 bases, Tm 62
SSR size	244 bases expected, Chinese Spring
Amplification Conditions	61C anneal, polymorphic on acrylamide (though weak).
Sequence	WMC411
Source Species	Triticum aestivum