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GrainGenes Probe Report: WMC412

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC412
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 10a6.1 Donor: Gale, John Innes Two inserts in this clone, this is the first insert. Trimmed to Rsa1 at 3' end, 5' end not located. Bases 459 to 475 show homology to 10f8 bases 112 to 128 (17 in longest run, maxScore = 26). (TTC)5 461 to 475, (GC)7 488 to 554, (GT)27 502 to 555.
Type	PCR SSR
PCR primers	Forward gATccccTccAAAAgTAgcATcT at 424 length 23 bases, Tm 64 Reverse cTTcAAcTgccTgcAcAcAAc at 563 length 21 bases, Tm 64
SSR size	160 bases expected, Chinese Spring
Sequence	WMC412
Source Species	Triticum aestivum