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GrainGenes Probe Report: WMC414

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC414  [ Marker Report ]
Locus	Xwmc414
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 23A1 Donor: Romestant, RAGT Trimmed to insert at both ends. Bases 88 to 109 (complement) show homology to 5d5.1 bases 46 to 67 (22 in longest run, maxScore = 21). Homology to one side of microsatellite. Bases 32 to 47 show homology to 24g6 bases 256 to 271 (complement) (16 in longest run, maxScore = 19). (AT)6 53 to 62, (GT)23 63 to 108, (GT)4 111 to 118.
Type	PCR SSR
PCR primers	Forward cgggTAAcAAAATAgTccATcc at 29 length 22 bases, Tm 61 Reverse TggTgTATTAgccgTAccAgAg at 146 length 22 bases, Tm 61
SSR size	139 bases expected, Chinese Spring
Amplification Conditions	tested 61C anneal, no product; tested 51C anneal, no product;
Sequence	WMC414
Source Species	Triticum aestivum