Query (optional)   in Class  All Allele Author Colleague Collection Assembly Gene Gene Class Gene Product Germplasm Image Journal Keyword Library Locus Map Map Data Marker Pathology Polymorphism Probe Protein QTL Rearrangement Reference Sequence Species Trait Trait Study Two Point Data  

---

GrainGenes Probe Report: WMC418

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC418  [ Marker Report ]
Locus	Xwmc418-3B Xwmc418 Xwmc418-3D wmc418 wmc418-3B
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 21d1 Donor: Langridge, CRC Australia Location from nullisomic/tetrasomic (Daryl Somers, AgCanada, Feb 2001) : 242 bases located on 3B, 264 bases located on 3B No trimming done to sequence at Agrogene, ends not located. Bases 56 to 555 show homology to 21g3 bases 58 to 557 (500 in longest run, maxScore = 423), duplicate, 21d1 has priority. (GT)13 295 to 320.
Type	PCR SSR
PCR primers	Forward AgAgcAgcAAgTTgTgTAgccA at 160 length 22 bases, Tm 65 Reverse TgAAgcTATTgccAgcAcgAg at 402 length 21 bases, Tm 65
SSR size	263 bases expected, Chinese Spring
Amplification Conditions	61C anneal, polymorphic on acrylamide (264-268 bases).
Sequence	WMC418
Source Species	Triticum aestivum