GrainGenes Probe Report: WMC420
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Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.
Probe
WMC420 [ Marker Report ]
Locus
Xwmc420-4A Xwmc420 Xwmc420-2A wmc420 wmc420-4A
Reference
Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks
Wheat Microsatellite Consortium Project 1, Plate reference 21a2 Donor: Langridge, CRC Australia Location from nullisomic/tetrasomic (Daryl Somers, AgCanada, Feb 2001) : 126 bases located on 4A Trimmed to insert at 3' end, 5' end not located. (GT)22 62 to 105, (GT)4 108 to 115.
Type
PCR SSR
PCR primers
Forward ATcgTcAAcAAAATcTgAAgTg at 15 length 22 bases, Tm 59
Reverse TTAcTTTTgcTgAgAAAAcccT at 118 length 22 bases, Tm 59
SSR size
125 bases expected, Chinese Spring
Amplification Conditions
51C anneal, polymorphic on acrylamide (120-124 bases)
Sequence
WMC420
Source Species
Triticum aestivum
GrainGenes Probe Report: WMC420
[Submit comment/correction][What is a probe?]
Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.
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