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GrainGenes Probe Report: WMC428

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC428  [ Marker Report ]
Locus	Xwmc428-3A Xwmc428 wmc428-3A wmc428
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 24a3 Donor: ITCF Location from nullisomic/tetrasomic (Daryl Somers, AgCanada, Feb 2001) : 259 bases located on 3A Trimmed to insert at both ends. Bases 166 to 263 (complement) show homology to 11g10.1 bases 378 to 475 (98 in longest run, maxScore = 86). (CA)25 186 to 235, (CA)4 247 to 254.
Type	PCR SSR
PCR primers	Forward TTAATccTAgccgTcccTTTTT at 39 length 22 bases, Tm 62 Reverse cgAccTTcgTTggTTATTTgTg at 274 length 22 bases, Tm 63
SSR size	257 bases expected, Chinese Spring
Amplification Conditions	51C anneal, polymorphic on acrylamide.
Sequence	WMC428
Source Species	Triticum aestivum