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GrainGenes Probe Report: WMC430

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC430  [ Marker Report ]
Locus	Xwmc430a [ Show all 11 ]
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 24d3 Donor: ITCF Trimmed to Ssp1 at 5' end and to insert at 3' end. Bases 161 to 195 (complement) show homology to 17b11 bases 59 to 93 (35 in longest run, maxScore = 54). Homology is on one side of microsatellite. (GT)10 55 to 74, (GT)4 79 to 86, (GT)14 131 to 158, (GT)5 249 to 258. Foward primer no match to 17B11, reverse primer last base does not match 17B11
Type	PCR SSR
PCR primers	Forward cAgTTgcAAgTTggccATAg at 16 length 20 bases, Tm 61 Reverse TAgggAccccTTgAcAAAAA at 160 length 20 bases, Tm 61
SSR size	164 bases expected, Chinese Spring
Amplification Conditions	51C anneal, polymorphic on acrylamide.
Sequence	WMC430
Source Species	Triticum aestivum