Query (optional)   in Class  All Allele Author Colleague Collection Assembly Gene Gene Class Gene Product Germplasm Image Journal Keyword Library Locus Map Map Data Marker Pathology Polymorphism Probe Protein QTL Rearrangement Reference Sequence Species Trait Trait Study Two Point Data  

---

GrainGenes Probe Report: WMC431

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC431  [ Marker Report ]
Locus	Xwmc431
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 24e3.1 Donor: ITCF Multiple inserts in this clone, this is the first insert. Trimmed to insert at both ends. Bases 181 to 221 show homology to 3f6 bases 197 to 237 (41 in longest run, maxScore = 70). Homology to one side of microsatellite. (GT)4 136 to 143, (CA)17 174 to 207.
Type	PCR SSR
PCR primers	Forward gcgAAgAccgATAATAAcAcAgA at 94 length 23 bases, Tm 62 Reverse TgcAAATgccATggTTATAgTAg at 260 length 22 bases, Tm 61
SSR size	189 bases expected, Chinese Spring
Amplification Conditions	61C anneal, stuttery smear.
Sequence	WMC431
Source Species	Triticum aestivum