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GrainGenes Probe Report: WMC432

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC432  [ Marker Report ]
Locus	Xwmc432-1D Xwmc432 wmc432
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 23a2 Donor: Romestant, RAGT Location from nullisomic/tetrasomic (Daryl Somers, AgCanada, Feb 2001) : 135 bases located on 4D, 191 bases located on 1D Trimmed to insert at 5' end and to Ssp1 at 3' end. (GT)14 238 to 265.
Type	PCR SSR
PCR primers	Forward ATgAcAccAgATcTAgcAc at 92 length 19 bases, Tm 53 Reverse AATATTggcATgATTAcAcA at 261 length 20 bases, Tm 54
SSR size	189 bases expected, Chinese Spring
Amplification Conditions	51C anneal, polymorphic on acrylamide (very complex).
Sequence	WMC432
Source Species	Triticum aestivum