Query (optional)   in Class  All Allele Author Colleague Collection Assembly Gene Gene Class Gene Product Germplasm Image Journal Keyword Library Locus Map Map Data Marker Pathology Polymorphism Probe Protein QTL Rearrangement Reference Sequence Species Trait Trait Study Two Point Data  

---

GrainGenes Probe Report: WMC435

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC435  [ Marker Report ]
Locus	Xwmc435 [ Show all 11 ]
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 24f4 Donor: ITCF Location from nullisomic/tetrasomic (Daryl Somers, AgCanada, Feb 2001) : 147 bases located on 5A Trimmed to insert at both ends. Bases 66 to 135 (complement) show homology to 5a4 bases 30 to 99 (70 in longest run, maxScore = 91) Homology on one side of microsatellite, forward primer has no homology to 5a4, reverse primer has a single mismatch. (GT)10 35 to 54,
Type	PCR SSR
PCR primers	Forward gcAcTATAcTTATTggATTgTcA at 4 length 23 bases, Tm 55 Reverse cATggTATcccTAgTAAgTTTTT at 127 length 23 bases, Tm 55
SSR size	146 bases expected, Chinese Spring
Amplification Conditions	51C anneal, smear.
Sequence	WMC435
Source Species	Triticum aestivum