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GrainGenes Probe Report: WMC436

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC436
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 24g4 Donor: ITCF Trimmed to insert at 5' end and to Ssp1 at 3' end. (CA)12 36 to 59.
Type	PCR SSR
PCR primers	Forward AATTggTAAgAcAcAcAgAAAc at 11 length 22 bases, Tm 55 Reverse TgcgTATgcATTcTATAgTAAA at 129 length 22 bases, Tm 55
SSR size	140 bases expected, Chinese Spring
Amplification Conditions	51C anneal, polymorphic on acrylamide.
Sequence	WMC436
Source Species	Triticum aestivum