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GrainGenes Probe Report: WMC437

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC437
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 24b5 Donor: ITCF Trimmed to insert at both ends. Bases 153 to 180 show homology to 11f8 bases 28 to 55 (28 in longest run, maxScore = 38). (GT)19 211 to 248. Bases 135 to 152 show homology to 15h3.3 bases 10 to 27 (18 in longest run, maxScore = 38).
Type	PCR SSR
PCR primers	Forward ATAgAggTTgTAcggccAAggT at 117 length 22 bases, Tm 63 Reverse AgTgTgAAgTAgcAgTcTATcccc at 253 length 24 bases, Tm 62
SSR size	160 bases expected, Chinese Spring
Amplification Conditions	61C anneal, polymorphic on acrylamide;
Sequence	WMC437
Source Species	Triticum aestivum