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GrainGenes Probe Report: WMC438

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC438  [ Marker Report ]
Locus	Xwmc438-7D
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 24f5 Donor: ITCF Location from nullisomic/tetrasomic (Daryl Somers, AgCanada, Feb 2001) : 261 bases located on 7D Trimmed to insert at both ends. (CA)17 106 to 139, (CA)9 142 to 159.
Type	PCR SSR
PCR primers	Forward gAccgTTgggcTgTATAgcATT at 8 length 22 bases, Tm 64 Reverse cTcTgAcAgTggTggAgcTTgA at 242 length 22 bases, Tm 65
SSR size	256 bases expected, Chinese Spring
Amplification Conditions	61C anneal, polymorphic on acrylamide.
Sequence	WMC438
Source Species	Triticum aestivum