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GrainGenes Probe Report: WMC442

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC442  [ Marker Report ]
Locus	Xwmc442
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 24g6 Donor: ITCF Location from nullisomic/tetrasomic (Daryl Somers, AgCanada, Feb 2001) : 142 bases located on 2B Trimmed to insert at 5' end and to Ssp1 at 3' end. Bases 256 to 271 (complement) show homology to 23A1 bases 32 to 47 (16 in longest run, maxScore = 19), homology on one side of microsatellite only. (CA)7 194 to 207, (CA)7 210 to 223, (CA)5 226 to 235, (AT)5 241 to 250.
Type	PCR SSR
PCR primers	Forward AcTAcTTTTTgcTTgAcAAcgA at 142 length 22 bases, Tm 58 Reverse AgTAAcAAAATAgTccAgcccT at 251 length 21 bases, Tm 58
SSR size	131 bases expected, Chinese Spring
Amplification Conditions	51C anneal, polymorphic on acrylamide.
Sequence	WMC442
Source Species	Triticum aestivum