Query (optional)   in Class  All Allele Author Colleague Collection Assembly Gene Gene Class Gene Product Germplasm Image Journal Keyword Library Locus Map Map Data Marker Pathology Polymorphism Probe Protein QTL Rearrangement Reference Sequence Species Trait Trait Study Two Point Data  

---

GrainGenes Probe Report: WMC443

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC443  [ Marker Report ]
Locus	Xwmc443 Xwmc443-5D wmc443 wmc443-5D
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 24a7 Donor: ITCF Location from nullisomic/tetrasomic (Daryl Somers, AgCanada, Feb 2001) : 205 bases located on 5D Trimmed to insert at 5' end and to Ssp1 at 3' end. (CT)44 62 to 149.
Type	PCR SSR
PCR primers	Forward ccTccTcTgTTTTcccTcTgTT at 14 length 22 bases, Tm 63 Reverse cAcAcTcTgTgcTTcTgTTTgc at 198 length 22 bases, Tm 63
SSR size	206 bases expected, Chinese Spring
Amplification Conditions	61C anneal, polymorphic on acrylamide.
Sequence	WMC443
Source Species	Triticum aestivum