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GrainGenes Probe Report: WMC444

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC444
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 24c7 Donor: ITCF Trimmed to insert at both ends. Bases 66 to 80 show homology to 22a11 bases 153 to 167 (15 in longest run, maxScore = 46) Homology on one side of microsatellite. (GT)10 225 to 244, (GT)9 247 to 264, (CT)9 207 to 224. Reverse primer is outside region of homology.
Type	PCR SSR
PCR primers	Forward gTTTcTTgTAAAccAcgccTTc at 77 length 22 bases, Tm 62 Reverse cAAcAAcTgATcATgAggAccA at 291 length 22 bases, Tm 63
SSR size	236 bases expected, Chinese Spring
Amplification Conditions	61C anneal, product.
Sequence	WMC444
Source Species	Triticum aestivum