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GrainGenes Probe Report: WMC446

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC446  [ Marker Report ]
Locus	Xwmc446 [ Show all 8 ]
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 24e7 Donor: ITCF Trimmed to insert at 5' end and to Ssp1 at 3' end. (CA)9 33 to 50, (CA)3 52 to 57. Too difficult to make primer.
Type	PCR SSR
PCR primers	Forward ccAgcTAgTAcTcTATATcTAcATc at 9 length 25 bases, Tm 52 Reverse TATTTgAAcAAgAgTTATgTgg at 103 length 21 bases, Tm 54
SSR size	115 bases expected, Chinese Spring
Amplification Conditions	51C anneal, stuttery product.
Sequence	WMC446
Source Species	Triticum aestivum