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GrainGenes Probe Report: WMC447

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC447
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 2A1 Donor: IGS BioTech Trimmed to insert at both ends. Bases 137 to 206 (complement) show homology to 24d1 bases 44 to 113 (70 in longest run, maxScore = 148). (GT)6 251 to 262, (GT)9 287 to 304. Bases 137 to 189 show homology to 26a5 bases 131 to 183 (complement) (53 in longest run, maxScore = 96). Near duplicate with 26a5, 2a1 has priority.
Type	PCR SSR
PCR primers	Forward AgAgATggATTggTAATTTTgT at 8 length 22 bases, Tm 56 Reverse TTcTcTccTTAggTTTgTTTTc at 319 length 22 bases, Tm 56
SSR size	333 bases expected, Chinese Spring
Amplification Conditions	51C anneal, product.
Sequence	WMC447
Source Species	Triticum aestivum