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GrainGenes Probe Report: WMC449

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC449
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 2c1.1 Donor: IGS BioTech Multiple inserts in this clone, this is the first insert. Trimmed to insert at 5' end and to Rsa1 at 3' end. (GA)32 50 to 113.
Type	PCR SSR
PCR primers	Forward gAgTggcAcccgTATATATggA at 22 length 22 bases, Tm 63 Reverse ggTgTcAAcAgcTcAccAgTcT at 123 length 22 bases, Tm 64
SSR size	123 bases expected, Chinese Spring
Amplification Conditions	61C anneal, polymorphic on acrylamide.
Sequence	WMC449
Source Species	Triticum aestivum