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GrainGenes Probe Report: WMC451

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC451
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 2h1.1 Donor: IGS BioTech Multiple inserts in this clone, this is the first insert. Trimmed to Ras1 at 5' end and to insert at 3' end. Bases 145 to 165 (complement) show homology to 11d6 bases 62 to 82 (21 in longest run, maxScore = 36). (GC)5 110 to 119, (AC)6 120 to 131.
Type	PCR SSR
PCR primers	Forward ccccgAgAAATcAgAAcAgAc at 4 length 21 bases, Tm 63 Reverse cTTgcTAgggTTTTgAgcgAg at 137 length 21 bases, Tm 63
SSR size	154 bases expected, Chinese Spring
Amplification Conditions	tested 61C anneal, no product; 51C anneal, polymorphic on acrylamide.
Sequence	WMC451
Source Species	Triticum aestivum