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GrainGenes Probe Report: WMC452

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC452
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 2b2 Donor: IGS BioTech Location from nullisomic/tetrasomic (Daryl Somers, AgCanada, Feb 2001) : 186 bases located on 5B, 196 bases located on 5D, 213 bases located on 5A Trimmed to Ras1 at 5' end and to insert at 3' end. (GCC)6 296 to 313.
Type	PCR SSR
PCR primers	Forward cgccTccTTcTggTTTATcAc at 230 length 21 bases, Tm 63 Reverse ccTTcTTccccTccAAATcTc at 393 length 21 bases, Tm 63
SSR size	184 bases expected, Chinese Spring
Amplification Conditions	61C anneal, product.
Sequence	WMC452
Source Species	Triticum aestivum