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GrainGenes Probe Report: WMC453

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC453  [ Marker Report ]
Locus	Xwmc453a-2A [ Show all 13 ]
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 2c2 Donor: IGS BioTech Location from nullisomic/tetrasomic (Daryl Somers, AgCanada, Feb 2001) : 172 bases located on 2A Trimmed to insert at 5' end and to Rsa1 at 3' end. (CA)35 119 to 188.
Type	PCR SSR
PCR primers	Forward AcTTgTgTccATAAccgAccTT at 73 length 22 bases, Tm 61 Reverse ATcTTTTgAggTTAcAAcccgA at 238 length 22 bases, Tm 61
SSR size	187 bases expected, Chinese Spring
Amplification Conditions	61C anneal, polymorphic on acrylamide (though complex).
Sequence	WMC453
Source Species	Triticum aestivum