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GrainGenes Probe Report: WMC456

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC456
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 2f4 Donor: IGS BioTech Trimmed to insert at 5' end and to Rsa1 at 3' end. Bases 103 to 278 show homology to 5g9 bases 240 to 415 (176 in longest run, maxScore = 124). (CA)16 103 to 134. The homology concerns just one side of the microsatellite.
Type	PCR SSR
PCR primers	Forward gggTTcAgTAAcgTAgccAgTg at 14 length 22 bases, Tm 63 Reverse ggAcATTTggATAAccgcTcTT at 165 length 22 bases, Tm 63
SSR size	173 bases expected, Chinese Spring
Amplification Conditions	tested 61C anneal, no product; 51C anneal, product -polymorphic around 320-360, possibly also 150-160.
Sequence	WMC456
Source Species	Triticum aestivum