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GrainGenes Probe Report: WMC458

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC458
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 2b6 Donor: IGS BioTech Trimmed to insert at both ends. Bases 160 to 182 (complement) show homology to 1C2 bases 332 to 354 (23 in longest run, maxScore = 40). (CA)18 183 to 218, (GA)4 219 to 226.
Type	PCR SSR
PCR primers	Forward gAATTgggcTAAAAcgTggA at 111 length 20 bases, Tm 62 Reverse AcTcTTTcgAccTcATggcg at 243 length 20 bases, Tm 64
SSR size	152 bases expected, Chinese Spring
Amplification Conditions	61C anneal, complex product.
Sequence	WMC458
Source Species	Triticum aestivum