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GrainGenes Probe Report: WMC459

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC459  [ Marker Report ]
Locus	Xwmc459
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 2d6 Donor: IGS BioTech Location from nullisomic/tetrasomic (Daryl Somers, AgCanada, Feb 2001) : 240 bases located on 4B Trimmed to Ras1 at 5' end and to insert at 3' end. (CA)14 186 to 213.
Type	PCR SSR
PCR primers	Forward cAgTgATcTTcTgggTgTTTTg at 9 length 22 bases, Tm 61 Reverse ATAAcgTgcAgcAcTcATcTcT at 233 length 22 bases, Tm 61
SSR size	246 bases expected, Chinese Spring
Amplification Conditions	61C anneal, polymorphic on acrylamide.
Sequence	WMC459
Source Species	Triticum aestivum