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GrainGenes Probe Report: WMC467

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC467
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 21g11 Donor: Dupont Trimmed to insert at 5' end and to Ssp1 at 3' end. Bases 78 to 107 (complement) show homology to 2f2 bases 66 to 95 (30 in longest run, maxScore = 38). (CA)27 128 to 181.
Type	PCR SSR
PCR primers	Forward cTAgTTgcgAgTcgATgggAAA at 79 length 22 bases, Tm 65 Reverse TATTcggATAgcAAccAggAgg at 183 length 22 bases, Tm 64
SSR size	126 bases expected, Chinese Spring
Amplification Conditions	61C anneal, product (60-78, 120-128).
Sequence	WMC467
Source Species	Triticum aestivum