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GrainGenes Probe Report: WMC470

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC470  [ Marker Report ]
Locus	Xwmc470 [ Show all 6 ]
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 25d1 Donor: Jizeng, Beijing Location from nullisomic/tetrasomic (Daryl Somers, AgCanada, Feb 2001) : 147 bases located on 3B Trimmed to insert at both ends. Bases 14 to 30 show homology to 5b5 bases 3 to 19 (17 in longest run, maxScore = 35). (CA)7 1 to 14, (CA)9 65 to 82, (CA)6 135 to 146.
Type	PCR SSR
PCR primers	Forward AcTTgcAAcTggggAcTcTc at 39 length 20 bases, Tm 61 Reverse TccccAATTgcATATTgAcc at 165 length 20 bases, Tm 62
SSR size	146 bases expected, Chinese Spring
Amplification Conditions	61C anneal, product.
Sequence	WMC470
Source Species	Triticum aestivum