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GrainGenes Probe Report: WMC472

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC472
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 25g1 Donor: Jizeng, Beijing Trimmed to insert at both ends. Bases 146 to 178 show homology to 17d10 bases 235 to 267 (33 in longest run, maxScore = 58). (CA)20 42 to 81,
Type	PCR SSR
PCR primers	Forward AcATTTcTcTccTAggTTTggTTTc at 4 length 25 bases, Tm 62 Reverse ATAAAAccTTggATggggATgA at 120 length 22 bases, Tm 63
SSR size	138 bases expected, Chinese Spring
Amplification Conditions	tested 61C anneal, no product. tested 51C anneal, no product.
Sequence	WMC472
Source Species	Triticum aestivum