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GrainGenes Probe Report: WMC475

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC475  [ Marker Report ]
Locus	Xwmc475-5A Xwmc475-7B wmc475 wmc475-5A wmc475-7B
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 25f3 Donor: Jizeng, Beijing Location from nullisomic/tetrasomic (Daryl Somers, AgCanada, Feb 2001) : 120 bases located on 5B, 132 bases located on 3A, 140 bases located on 5A, 156 bases located on 7B Trimmed to insert at 5' end and to Ssp1 at 3' end. (GT)21 292 to 333.
Type	PCR SSR
PCR primers	Forward AAcAcATTTTcTgTcTTTcgcc at 206 length 22 bases, Tm 62 Reverse TgTAgTTATgcccAAccTTTcc at 338 length 22 bases, Tm 62
SSR size	154 bases expected, Chinese Spring
Amplification Conditions	61C anneal, product (1 sample failed in PCR).
Sequence	WMC475
Source Species	Triticum aestivum