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GrainGenes Probe Report: WMC476

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC476  [ Marker Report ]
Locus	Xwmc476-7B Xwmc476 wmc476 wmc476-7B
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 25h3 Donor: Jizeng, Beijing Location from nullisomic/tetrasomic (Daryl Somers, AgCanada, Feb 2001) : 204 bases located on 7B Forward sequence only. Trimmed to insert at both ends. Bases 202 to 235 show homology to 9e5 bases 183 to 216 (34 in longest run, maxScore = 83). Homology to one side of microsatellite. Reverse primer three mismatches to 9e5. (GT)7 118 to 131, (GT)25 134 to 183.
Type	PCR SSR
PCR primers	Forward TAccAAccAcAccTgcgAgT at 90 length 20 bases, Tm 63 Reverse cTAgATgAAccTTcgTgcgg at 270 length 20 bases, Tm 63
SSR size	200 bases expected, Chinese Spring
Amplification Conditions	tested 61C anneal, no product. tested 51C anneal, no product.
Sequence	WMC476
Source Species	Triticum aestivum